What was the selectable marker?

/in /by

DO NOT DO RESULT SECTION 

This lab report will cover the transformation of E.coli  and the restriction digest of the plasmid (labs 5 to 8).

Use the Standard lab report format and include the following 

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– Overall objective 

– Background to what you were trying to do 

– Methods – used to create reagents and used for the transformation, plasmid isolation, restriction digest and agarose gels.  Create sub section for each 

Transformation

– What was the selectable marker?

– How do you know you successfully transformed the cells with pGlo

– What was the most critical step in the transformation process

Plasmid preparation

– Why does only the E. coli grown in ara/amp fluoresce

Restriction digest 

– What size fragments would you expect when cutting the plasmid with the two enzymes used? 

– Are the results of the plasmid what you expected.

– Are the two fragments the sizes you expected ?

 

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