For the optimal pH experiment, which enzyme/substrate pair did you use?
1. For the optimal pH experiment, which enzyme/substrate pair did you use? List your results for the absorbance at one minute when the pH was 2, 4, 6, 8 and 10. Based on your results, what is the optimal pH for this enzyme?
2. For the optimal temperature experiment, which enzyme/substrate pair did you use? List your results for the absorbance at one minute when the temperature was 10, 20, 30, 40, 50, 60, 70 and 80 degrees. Based on your results, what is the optimal temperature for this enzyme?
3. For the reaction rates experiment, in the space below, record the absorbance values you obtained at 30 seconds, 1 minute, 1 1/2 minutes and at 2 minutes for 5 and 15 mls of enzyme. How does enzyme concentration influence the rate of reaction? Why?
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