The higher education department of Holmes Institute recorded data on the number of students enrolled in the different study majors for the years 2018 and 2019. The data are stored in file STUDYMAJOR.xls. a) Use an appropriate graphical technique or chart to compare the number of enrolments in 2018 and 2019 of the different study majors. Display the chart. (2 marks) b) Use an appropriate graphical technique or chart to display the percentage value of the number of enrolment of the different study major in 2018 and 2019. Display the chart. (2 marks) Note: Questions 2 to 6 are related. Question 2 (8 marks) Sociologists argued that women on average earn less than men as women often choose to work less hours. They further suggest that the choice of hours worked may be driven by various factors such as age, childcare needs, occupation choice and flexibility. To investigate the relation between hours worked and income earned by Australian men and women, a researcher plans to survey a sample of individuals across the country. Briefly explain (using no more than 250 words in total for this question) a) What type of survey method the researcher could use and why? (2 marks) b) What sampling method could the researcher use to select his/her sample and why? (2 marks) c) What are the two main variables the researcher should consider collecting data for the purpose of the above analysis and why? Identify the data type(s) for the variables. (2 marks) d) What kind of issues the researcher may face in this data collection? (2 marks) Suppose a researcher has collected data from a sample of 65 individuals using the sampling method you have proposed in (b). For each individual, the hours worked per week and yearly income (measured in ‘000’s dollars) were recorded. The data are stored in file HOURSWORKED.xls.

Attachments:

Stastics-for-….docx

STUDYMAJOR.xls

HOURSWORKED.xls

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You will be provided with a case study, comprising a trial balance and additional information, in relation

to a fictitious company. This case study can be accessed from the course learnonline site. Using this

information as a basis, you are required to prepare an annual report suitable for publication a particular

year ending 30 June. This must include the following:

? title page and contents page

? statement of profit or loss and other comprehensive income

? statement of financial position

? statement of changes in equity

? notes to the financial statements

? directors declaration

? annual directors report

? annual auditors report

? reference list.

These must meet the minimum requirements of those Australian accounting standards/

pronouncements and corporations law as detailed in this booklet.

In addition you must include a Statement on Use of Draft Feedback. Further details about this is

included later in this document.

Attachments:

FA2-Annual-Re….pdf

Assignment-Ch….jpg

Handout-FA2-A….pdf

FA2-Assignmen….docx

ACCT2005-SP2-….pdf

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Question 1: Explain how map kinase is phosphorylated in response to NGF stimulation (maximum 200 words) Question 2: Explain the principles of how Western blotting works. (no maximum on each of these sections but each section should be able to be answered in a couple of sentences) I. Why are the proteins in the PC 12 cell extracts incubated with ß-mercaptoethanol, sodium dodecyl sulphate (SDS) and then heated to 100°C II. What is the basis of the separation of proteins by SDS gel electrophoresis III. Why is the gel incubated with transfer buffer prior to electrophoretic transfer IV. Why do we transfer the proteins to the solid support, the nitrocellulose filter? Why can’t the antibody detection of map kinase be done when it is still in the gel? V. After the proteins have been transferred to the nitrocellulose membrane why is the nitrocellulose membrane incubated in blocking buffer prior to addition of the two different primary antibodies? VI. What form/s of map kinase does the anti-total map kinase antibody detect? VII. What form/s of map kinase does the anti-active map kinase antibody detect? VIII. Why does an intense purple colour develop on the nitrocellulose membrane at the position of the map kinase molecules? Question 3: What do the results of the experiment that are presented by Sam at the end of the video tell you about the effect of NGF stimulation of PC12 cells on the level of total map kinase and the level of map kinase that is phosphorylated and active? (maximum 200 words) Question 4: Why are there map kinase proteins with different molecular weight? (maximum 200 words)

Attachments:

Intracellular….docx

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A child comes into the clinic and is suspected to have Cushing syndrome. Which test(s) could be utilized to determine cortisol levels in this patient? Select all that apply. 1. Fasting blood glucose. 2. Thyroid panel (TSH, T3, T4). 3. 24-hour urine for 17-hydroxycorticoids. 4. Radiographic studies of the bones. 5. Cortisone suppression test. 6. Urine culture. 7. Complete blood count.

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